FluorCamTsarin daukar hoto na tebur na shuka-shuka mai yawa

- Kayan aikin fasahar da aka fi amfani da shi a cikin binciken tsire-tsire na tsire-tsire da binciken gwaje-gwaje na muhalli

PSIBabban masanin kimiyya na kamfanin, Farfesa Nedbal da Shugaban kamfanin, Dr. Trtilek da sauransu, sun fara haɗuwa da fasahar fluorescence ta PAM tare da fasahar CCD don haɓaka da samar da tsarin hoton fluorescence na FluorCam a duniya a 1996 (Heck et al., 1999; Nedbal da sauransu, 2000; Govindjee and Nedbal, 2000）。 FluorCam chlorophyll fluorescence imaging fasaha ya zama wani muhimmin ci gaba a cikin 1990's chlorophyll fluorescence fasaha, sa masana kimiyya a kan photosynthesis da binciken chlorophyll fluorescence nan da nan shiga 2D duniya da kuma microscopic duniya. A halin yanzu, kamfanin PSI ya zama mafi iko, mafi yawan amfani, mafi cikakken nau'i, mafi yawan takardun da aka buga na chlorophyll fluorescence imaging masana'antun masana'antun duniya

Hoton sama da hagu shine fasahar daukar hoto ta FluorCam da Nedbal da sauransu suka tsara a shekarun 1990 (Binciken Photosynthesis, 66: 3-12, 2000) da dama shine hoton launi na lemon da hoton daukar hoto ta chlorosynthesis (Photosynthetica, 38: 571-579, 2000)

FluorCamDesktop shuka Multi-spectrum fluorescent imaging tsarin ne mai tsananin hadewa, sosai m, sauki don amfani, da kuma amfani da fadi na high-karshen shuka live imaging fasahar kayan aiki, high m CCD ruwan tabarau, 4 m LED haske tushen allon da kuma kula da tsarin da sauransu hadedde a cikin wani duhu daidaitawa aiki akwatin (kuma za a iya zaɓar da biyar haske tushen allon sanya a saman bisa ga bukatun), shuka samfurin sanya a kan allon a cikin duhu daidaitawa aiki akwatin, allon matakin 7 tsawo daidaitawa; Haske tushen samar da wutar lantarki ta hanyar high kwanciyar hankali samar da rukuni, 4 high makamashi, high kwanciyar hankali LED haske tushen allon uniformly haskaka a kan shuka samfurin, imaging yankin iya zuwa 13×13 cmTsarin sarrafawa yana haɗe da kwamfuta ta hanyar USB kuma yana sarrafawa da tattara bayanan bincike ta hanyar shirye-shiryen FluorCam software. Ana amfani da shi don wasu tsire-tsire kamar ganye na tsire-tsire da 'ya'yan itace, dukan tsire-tsire ko tsire-tsire da yawa da aka noma, tsire-tsire da ƙananan tsire-tsire, algae da sauransu, ana amfani da shi sosai a cikin tsire-tsire ciki har da algae photosynthesis physiological muhalli, tsire-tsire matsala tilasta physiology da sauƙi, porous aiki, tsire-tsire muhalli kamar ƙasa mai nauyi karfe gurɓataccen amsa da kuma binciken halitta, tsire-tsire juriya ganowa da kuma binciken, amfani

Main fasali:

· Tsarin da aka haɗa a cikin akwatin aiki na duhu mai daidaitawa, mai sauƙin aiki da sauƙin motsi, ana iya yin nazarin auna hoton duhu mai daidaitawa a cikin dakin gwaje-gwaje ko waje

· High hankali CCD ruwan tabarau, lokaci ƙuduri har zuwa 50 hotuna a kowane dakika, sauri kama chlorophyll fluorescent transient, hoto yankin har zuwa 13x13cm

· Yana da kawai duniya iya gudanar da OJIP sauri fluorescence dynamic imaging nazarin high-karshen chlorophyll fluorescence fasahar kayan aiki, iya samun OJIP sauri chlorophyll fluorescence dynamic curve da Mo (OJIP curve farko inclination), OJIP m yanki, Sm (ma'auni na makamashi da ake bukata don rufe duk haske amsa cibiyoyin), QY, PI (Performance Index) da sauransu fiye da 20 sigogi

· ne kawai high-karshen chlorophyll fluorescence fasahar kayan aiki a duniya iya gudanar da QA re-oxidation dynamic imaging nazarin, iya gudanar da daya-juyawa cike haske flash (STF) chlorophyll fluorescence induced dynamics, haske karfi a100 m120,000 μmol (photons) / m².s a cikin

· Tare da mafi aiki, editable Chlorophyll fluorescence gwaji shirye-shirye (Protocols), ciki har da snapshot yanayin, Fv / Fm, Kautsky induced tasiri, 2 Chlorophyll fluorescence kashewa nazarin (NPQ) protocols (2 saiti na musamman ga haske shirye-shirye), LC haske amsa curve, PAR sha da NDVI hoto nazarin, QA re-oxidation dynamics nazarin (zaɓi), OJIP sauri fluorescence dynamics nazarin (zaɓi), da GFP kore fluorescence furotin hoto (zaɓi), da sauransu

· Za a iya gudanar da atomatik maimaita image ma'auni nazarin, tsoho gwaji tsari (Protocols), yawan ma'auni da kuma tsawon lokaci, tsarin zai ta atomatik zagaye gudanar da image ma'auni, da kuma ta atomatik ajiye bayanai a kwamfuta da lokaci kwanan wata (tare da lokaci hatimi); Har ila yau, biyu gwaji shirye-shirye (Protocols) za a iya tsara; Misali don yin tsarin ta atomatik gudanar da Fv / Fm a rana, ta atomatik gudanar da nazarin NPQ da sauransu da dare

· Tare da launi biyu photochemical haske motsawa haske tushen, misali daidaitawa don ja da fari, zaɓi da ja da shuɗi da sauran biyu-band photochemical haske, biyu launi photochemical haske za a iya amfani da shi a daban-daban rabo, don gwaji daban-daban haske inganci a kan amfanin gona / shuka photosynthesis amfanin.

Hagu Figure A ne Fv / Fm na cucumber ganye a karkashin 100% ja haske yanayi, hagu Figure B ne Fv / Fm na cucumber ganye a karkashin 30% shuɗi haske yanayi; A saman dama zane ne dangantaka tsakanin photosynthesis ƙarfi da haske ƙarfi (daban-daban rabo na blue haske), da kuma a ƙasa dama zane ne dangantaka tsakanin air hole conductivity da haske ƙarfi (daban-daban rabo na blue haske)

· Mai aiki Chlorophyll fluorescence imaging, Multi-spectrum fluorescence imaging, GFP daidaitaccen halin fluorescence imaging

· Na zaɓi tare da TetraCam launi imaging module, max hoto yankin 20x25cm don ganye ko shuka siffofin hoto nazari da kuma chlorophyll fluorescence hoto kwatance nazari

· Za a iya zaɓi tare da high spectrum imaging na'ura da kuma infrared thermal imaging na'ura, shuka halaye dijital, gani, cikakken ma'auni nazarin shuka siffofin, photosynthesis inganci, biochemical halaye, iska hole conductivity, tilasta da juriya da sauransu

· Ana iya yin amfani da babban tsarin nazarin hoton shuka mai motsi, yankin hoton 35x35cm, yana aiki da hoton fluorescent na chlorophyll, hoton zafi na infrared da nazarin hoton RGB

latest aikace-aikace:

Hendrik KupperAn buga shi tare da Zuzana Benedikty da sauransu a watan Fabrairu na 2019 Analysis of OJIP Chlorophyll Fluorescence Kinetics and QA Reoxidation Kinetics by Direct Fast Imaging， Binciken ya fara amfani da Ultra-high gudun daukar hoto firikwensin FluorCam tebur shuka chlorophyll fluorescence daukar hoto tsarin da FKM multi-spectrum microfluorescence daukar hoto tsarin, daukar hoto gudun 4000fps@640x512 ， QA Reoxidation Chlorophyll Fluorescent Dynamic Imaging auna guda pulse cike haske flash150,000mmol / m2sa1.

Attachments: OJIP sauri fluorescence dynamics ƙididdiga bincike sigogi sun haɗa da:

a)da: farko fluorescence ko kira mafi ƙarancin fluorescence, fluorescence a 50μs

a)da: Fluorescence a lokacin 2ms

a)Mai: Fluorescence a 60ms

da)PKo Fm: mafi girma fluorescence

a)da= (Fj-Fo) / (Fm-Fo): j matakin fluorescence dangane canji

a)da= (Fi-Fo) / (Fm-Fo): I-matakin fluorescence dangane masu canji

a)Mai= TRo / RC-ETo / RC = 4 (F300-Fo) / (Fm-Fo): Fluorescent transient farkon karkatarwa, ko da ake kira OJIP curve farkon karkatarwa

a)Yankin: Yankin tsakanin OJIP curve da Fm, wanda za a iya kira yankin daidaitawa don kwatanta samfuran daban-daban, yankin yana buƙatar daidaitawa zuwa: Sm = Yankin / (Fm-Fo), Sm shine ma'aunin makamashi da ake buƙata don rufe duk cibiyoyin amsawar haske

a)gyara Area: Fixed yankin OJIP, yankin da ke ƙasa da F darajar a lokacin da OJIP curve 40 m zuwa 1 second a lokacin da F darajar

a)SM: daidaitaccen OJIP diyya yankin, yana nuna QA sake dawowa da yawa juyawa

a)da= Vj / Mo: daidaitattun OJ mataki diyya yankin, nuna guda juyawa QA sake dawowa

a)N = Sm / Ss = Sm Mo (1 / Vj): OJIP QA mayar da yawan juyawa (tsakanin 0 da t_Fm）

m)Phi-Po＝QY＝φpo＝TRo/ABS＝Fv/Fm， Max haske quantum samarwa, sha haske quantum kwarara reaction cibiyar farko kama rabo

a)Psi_o＝ψo＝ETo/TRo＝1-Vj， Rashin kwararar kwararar kwararar kwararar kwararar kwararar kwararar kwararar kwararar kwararar kwararar kwararar kwararar kwararar kwararar kwararar kwararar kwararar kwararar kwararar kwararar kwararar kwararar

a)Phi_Eo＝φ_kuma=ETo/ABS=(1-(Fo/Fm))(1-Vj)， Quantum yield na lantarki ta hanyar lantarki ta hanyar lantarki ta hanyar lantarki ta hanyar lantarki ta hanyar lantarki ta hanyar lantarki ta hanyar lantarki ta hanyar lantarki ta hanyar lantarki ta hanyar lantarki ta hanyar lantarki ta hanyar lantarki ta hanyar lantarki ta hanyar lantarki

m)Phi_Do＝φ_da＝1-φpo＝Fo/Fm， Makamashi watsa haske quantum samarwa (t = 0)

m)Phi_pav= φpav = φpo (Sm / t)_Fm), matsakaicin haske quantum samarwa, t_FmLokaci da ake bukata don cimma Fm (ms)

a)ABS / RC= Mo ((1 / Vj) ((1 / QY) : don sha haske quantum kwarara na unit amsa cibiyar, a nan amsa cibiyar kawai nufincibiyoyin aiki (QA zuwa QA) rage(Haka kuma). QY=TRo/ABS=Fv/Fm

sa)TRo / RC= Mo ((1 / Vj): farkon (ko mafi girma) kama haske quantum kwarara na Unit Amsa Cibiyar (wanda ke haifar da rage QA, watau karuwar rabo na rufe cibiyar amsa B)

ta)ETo / RC= Mo (1 / Vj) (1-Vj): Ƙungiyar Amsa Cibiyar Bayar da Lantarki ta Farko

a)DIo / RC= (ABS / RC) - (TRo / RC): Rashin makamashi na cibiyar amsawa

da)ABS / CS: sha haske quantum kwarara na unit samfurin sashe,CS yana tsaye don sashin ƙwanƙwasa mai ban sha'awa na samfurin da aka gwada(Haka kuma). ABS / CSo = Fo, ABS / CSm = Fm, TRo / CSx = QY (ABS / CSx) - Unit sashe kama makamashi ko haske quantum gudu

m)TSO / TSO= YY. ETo / CSo = φ_kuma.F = YY. (1-Vj). da

da)RC / CSx: Amsa cibiyar yawa,RC / CS0 (RCs masu aiki a kowace sashe mai motsawa)

a)PI_ABS=(RC/ABS) (φpo/φ)_da(ψo / Vj): Index na "aiki" ko Index na rayuwa bisa sha kwararar haske

a)PICs=(RC/CSx) (φpo/φ)_da(ψo / Vj): ƙididdigar "aiki" ko ƙididdigar rayuwa bisa sashe