FluorCam rufe GFP / Chl. Tsarin hoto na fluorescence

FluorCamTsarin daukar hoto na GFP / Chl. mai rufewa na iya ba da sabis ga jerin binciken kwayoyin halitta da ƙwayoyin halitta waɗanda aka yi amfani da su ga sunadarai masu ƙwayoyin halitta masu ƙwayoyin halitta masu ƙwayoyin halitta masu ƙwayoyin halitta masu ƙwayoyin halitta masu ƙwayoyin halitta masu ƙwayoyin halitta. An shigar da matattarar matattarar da aka sarrafa ta hanyar software da kuma lantarki a kan tsarin daukar hoto na rufewa, tare da jerin matattarar matattarar da za a iya ganowa da hoto na GFP, EGFP, wtGFP, YFP, BFP ko wasu sunadarai da fluorescents.

FluorCam rufe GFP / Chl. fluorescence imaging tsarin kuma za a iya amfani da gano m canje-canje da kuma sararin samaniya rarraba na shuka fitar da fluorescence, auna Kautsky tasirin tsari, fluorescence kashewa da sauran lokaci fluorescence tsari (transient) da kuma samar da 2D fluorescence hotuna. Kayan aikin da aka tsara musamman yana ba da damar canza tsarin ta atomatik zuwa fluorescent protein, fluorescent imaging ko fluorescent ganowa na chlorophyll. Tsarin yana da haɗin kai sosai kuma ana iya daidaitawa da samfurin duhu.

Aikace-aikace:

· Fluorescence ganowa na fluorescent furotin da fluorescents

· Canja wurin kwayoyin halitta da fasahar biosensor

· Binciken tsire-tsire na GMO

· Daban-daban genome juriya bincike

· Nazarin hulɗar tsire-tsire-tsire

· Nazarin hulɗar shuka-dabbobi na asali

· Plant photosynthesis halaye da kuma metabolic disorders screening

· Ganowa na halitta da kuma non-halitta tilasta

· tsire-tsire resistance ko m bincike

· Binciken rashin daidaito na iska

· Nazarin rikice-rikice na metabolism

· Tsarin ƙarfi da samarwa

Ayyuka:

· Easy aiki, auna samfurin height daidaitacce, hadewa da keɓaɓɓun duhu daidaitawa akwatin, sauki duhu daidaitawa aiki, aiki da sauransu na tsire-tsire da aka gwada

· High ƙuduri CCD, ƙuduri har zuwa 1392 × 1040 pixels, sauri daidai kama chlorophyll fluorescence da GFP

· Za a iya yin ta atomatik maimaita image ma'auni, za a iya saita wani gwaji shirin (Protocols) ta atomatik zagaye image ma'auni, image ma'auni bayanai ta atomatik ajiye a kwamfuta ta hanyar lokaci kwanan wata (tare da lokaci hatimi)

· Tare da Kautsky induction tasiri, fluorescence kashewa bincike da sauransu daban-daban m gwaji tsari (protocols), auna bincike sigogi har zuwa fiye da 50

· Zaɓi tare da TetraCam launi imaging module, max imaging yankin 20 × 25cm don hasken ko shuka siffar auna bincike

Typical samfurin:

• ganye, tsire, tsire, tushe, dukan ƙananan tsire-tsire

• Healing tissue, ƙananan tsire-tsire a cikin Petri kwanoni

• 'Ya'yan itace da furanni

• Blue kwayoyin cuta, Green algae, kwayoyin cuta

• Dabbobi masu canjin kwayoyin halitta

fasaha sigogi:

• auna sigogi: Fo, Fo’, Fs, Fm, Fm’, Fp, FtDn, FtLn, Fv, Fv'/ Fm'，Fv/ Fm ,Fv',Ft,ΦPSII,NPQ_Dn, NPQ_Ln, Qp_Dn, Qp_Ln, qN, qP，QY, QY_Ln,Rfd, ETR Fiye da 50 chlorophyll fluorescence sigogi, da dai sauransu, kowane sigogi zai iya nuna 2D fluorescence launi image

· Da cikakken atomatik auna shirye-shirye (protocol), kyauta gyara atomatik auna shirye-shirye

a) Fv / Fm: auna sigogi sun hada da Fo, Fm, Fv, QY da sauransu

b) Kautsky induction tasiri: Fo, Fp, Fv, Ft_Lss, QY, Rfd da sauran sigogin fluorescence

c) Fluorescent kashewa bincike: Fo, Fm, Fp, Fs, Fv, QY, ΦII, NPQ, Qp, Rfd, qL da sauransu fiye da 50 sigogi

d) Light amsa curve LC: Fo, Fm, QY, QY_Ln, ETR da sauransu fluorescent sigogi

e) PAR sha tare da NDVI (zaɓi)

f) A Oxidation Dynamics (zaɓi)

g) Masu auna hoton fluorescence kamar GFP

h) OJIP sauri fluorescence dynamics analysis (zaɓi): Mo (OJIP curve farko slope), OJIP m yanki, Sm (ma'auni na makamashi da ake buƙata don kashe duk haske amsa cibiyoyin), QY, PI da sauransu 26 sigogi

§ High ƙuduri TOMI-2 CCD firikwensin

a) Binciken layi-layi na CCD

b) Matsakaicin ƙudurin hoto: 1360 × 1024 pixels

c) Lokaci ƙuduri: har zuwa 20 frames a kowane dakika a kan mafi girma image ƙuduri

d) A / D juyawa ƙuduri: 16bit (65,536 launin toka mataki)

e) Abubuwan girma: 6.45μm × 6.45μm

f) Yanayin aiki: 1) Yanayin bidiyo mai mahimmanci don auna sigogin fluorescence na chlorophyll; 2) Snapshot yanayin don auna fluorescent furotin da fluorescent launi kamar GFP

g) Sadarwa Mode: Gigabit Ethernet

• Hasken tushen allon: 4 babban babban karfi kunshin LED hasken tushen allon, kowane hasken tushen allon ya ƙunshi 8 × 9 LEDs, hasken tushen allon yana da tasiri yanki iri daya da hoton yanki 13 × 13cm

• Ma'auni haske: misali tare da 617nm ja haske, sauran bands zaɓi, tsawon 10μs-100μs daidaitacce

• Bi launi photochromatic haske: 2 ja haske (617nm) + 2 blue haske (470nm), Actinic1 Max haske karfi 300µmol (photons) / m².s, Actinic2 Max haske karfi 2000µmol (photons) / m².s; Max photochemical haske za a iya haɓaka zuwa 3000 μmol (photons) / m².s

• Hasken wuta: Max hasken ƙarfi 4000μmol (photons) / m².s, za a iya haɓaka zuwa 6000 μmol (photons) / m².s

• Yankin hoto: 13 × 13cm, Za a iya bincika hoton ganye-ganyen tsire-tsire, tsire-tsire, algae, moss, ƙasa, tsire-tsire ko tsire-tsire da yawa, 96 rami, 384 rami, da dai sauransu

• GFP ma'auni module: 7-bit tace ƙafafun da keɓaɓɓun tace don auna GFP (kore fluorescent furotin)

• PAR sha ma'auni module (zaɓi): nisan ja haske 740nm (FAR), 660nm biyu launi LEDs haske tushen allon da keɓaɓɓun tace don auna PAR sha da NDVI

• QA a cikin Oxidation Dynamics Imaging Analysis (zaɓi): Za a iya yin STF fluorescence Dynamics Analysis ma'auni tare da daya-zagaye haske flash (STF) haske karfi har zuwa 120000 μmol (photons) / m².s in100μs

• OJIP Fast Fluorescent Dynamics Module (Zaɓi): Lokaci ƙuduri har zuwa 1μs, ƙididdigar ƙuduri na OJIP tare da sigogi ashirin da suka dace ciki har da: Fo, Fj, Fi, P ko Fm, Vj, Vi, Mo, Area, Fix Area, Sm, Ss, N (QA rage yawan juyawa), Phi‐‐_Po, Psi_o, Phi_Eo, Phi_Do, Phi_pav, ABS / RC (da sha haske quantum gudu a Unit Response Center), TRo / RC (da fara kama haske quantum gudu a Unit Response Center), ETo / RC (da fara lantarki watsa haske quantum gudu a Unit Response Center), DIo / RC (da Unit Response Center makamashi watsa), ABS / CS (da sha haske quantum gudu a Unit samfurin sashe), TRo / CSo, RC / CSx (Response Center yawa), PIABS (da "yi" index ko da ake kira rayuwa index bisa sha haske quantum gudu), PIcs (da "yi" index ko da ake kira rayuwa index bisa sashe)

• UV LED haske tushen allon (zaɓi): 365nm ko 385nm, 1) auna Multi-launi fluorescence (duba FluorCam Multi-spectrum fluorescence imaging tsarin cikakken bayani); 2) auna wtGFP (daji irin kore fluorescent furotin), DAPI (4 ', 6-dimethyl-2-phenylpiperine, wani fluorescent launi)

• Blue LED haske tushen allon (zaɓi): 470nm, 1) auna EGFP (ingantaccen kore fluorescent furotin); 2) Ana amfani da Air Hole aiki bincike

• Green LED haske tushen allon (zaɓi): 530nm, auna YFP (rawaya fluorescent furotin)

* Ma'auni wtGFP, DAPI, EGFP, YFP da sauran fluorescent furotin ko fluorescent launi kuma yana buƙatar zaɓin dacewa tace

• FluorCam Chlorophyll Fluorescent Imaging Analysis Software aiki: tare da Live (Live gwaji), Protocols (gwaji shirye-shiryen zaɓi keɓaɓɓu), Pre-processing (imaging pre-processing), Sakamakon (imaging analysis sakamakon) da sauransu aiki menu

• Abokin ciniki musamman gwaji shirye-shirye yarjejeniya (protocols): Za a iya saita lokaci (kamar auna haske tsawon lokaci, photochemical haske tsawon lokaci, auna lokaci, da dai sauransu), haske karfi (kamar daban-daban haske ingancin photochemical haske karfi, saturated haske karfi, modulation auna haske, da dai sauransu), tare da keɓaɓɓun gwaji shirye-shirye harshe da rubutun, masu amfani kuma iya samun kyauta don ƙirƙirar sabon gwaji shirye-shirye ta amfani da wizard shir

• Auto auna analytical aiki: Za a iya saita wani gwaji shirin (Protocol) ta atomatik unmanned zagaye image auna, maimaitawa da kuma tsakanin lokaci abokin ciniki musamman, image auna bayanai ta atomatik ajiye a kwamfuta ta hanyar lokaci kwanan wata (tare da lokaci hatimi)

  Snapshot yanayin: Ta hanyar snapshot hoto yanayin, za a iya kyauta daidaita haske ƙarfi, shutter lokaci da kuma hankali samun m shuka samfurin steady halin fluorescence da nan take fluorescence hotuna

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• Pre-imaging: Software na shirin zai iya gano samfuran tsire-tsire da yawa ko yankuna da yawa ta atomatik, kuma zai iya zaɓar yankin sha'awa (ROI) da hannu. Cikakken zaɓin hannu na iya zama murabba'i, zagaye, wani polygon ko ɓangare. Software auna ta atomatik bincike fluorescence dynamics curves da daidai sigogi na kowane samfurin da kuma zaɓi yanki, samfurin ko yanki adadin Unlimited (> 1000)

• Bayanan bincike yanayin: tare da "siginar lissafi recalculation" yanayin (arithmetic matsakaicin) da kuma "siginar matsakaicin recalculation" yanayin, zaɓi "siginar lissafi recalculation" yanayin a cikin yanayin high siginar amo rabo, zaɓi "siginar matsakaicin recalculation" yanayin a cikin yanayin low siginar amo rabo don tace kuskuren da amo ya kawo

• Output sakamakon: High lokaci ƙuduri fluorescence dynamic chart, fluorescence dynamic canji video, fluorescence sigogi Excel fayil, histogram, daban-daban sigogi hoto chart, daban-daban ROI fluorescence sigogi jerin da sauransu

• CCD mai ganowa bandwidth: 400-1000nm

· Bayar da haske tsarin: tsayayye ko m (sinus irin)

· Spectrum amsa: mafi girma quantum inganci (70%) a 540nm, 50% sauya a 400nm da 650nm

· Karanta amo: kasa da 12eRMS, yau da kullun 10e

· Cikakken ƙwarewar tarko: fiye da 70,000 e (unbinned)

· Bios: Firmware da za a iya haɓaka

· Hanyar sadarwa: Gigabit Ethernet

· Girma: 471 mm (W) × 473 mm (D) × 512 mm (H)

· Nauyi: kimanin 40 kg

· Shigar da wutar lantarki: Apr. 1100 W

· Wutar lantarki: 90-240 V

Typical aikace-aikace:

Cibiyar Kimiyyar Rayuwa ta Shanghai ta yi amfani da FluorCam don auna hoton hoton GFP da aka samu. Tsire-tsire masu launi mai haske a cikin hoton suna nuna GFP.

Button Coral shrinkage - fadada ba tare da lokaci ba (T1 = 0, T2 = 30, T3 = 60) Binciken hoton NDVI da GFP (Miguel, 2014)

Asalin: Turai